Characterization of a cDNA encoding a human kidney, cytochrome P-450 4A fatty acid omega-hydroxylase and the cognate enzyme expressed in Escherichia coli

Biochim Biophys Acta. 1993 Feb 20;1172(1-2):161-6. doi: 10.1016/0167-4781(93)90285-l.

Abstract

A cDNA encoding a cytochrome P-450 4A (CYP4AII) was cloned from a human kidney cDNA library. Northern blot analysis and RNase protection assays indicate that related mRNAs occur in kidney and liver with the highest abundance found in kidney. The enzyme was expressed from its cDNA in Escherichia coli. A solubilized preparation of the enzyme reconstituted with cytochrome P-450 reductase catalyzed the omega-hydroxylation of lauric acid, palmitic acid, and arachidonic acid with turnover numbers of 9.8, 2.2 and 0.55 min-1, respectively. Little or no activity was detected toward prostaglandins A1 and E1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular / methods
  • Cytochrome P-450 CYP4A
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • DNA / genetics*
  • Escherichia coli / genetics*
  • Humans
  • Kidney / enzymology*
  • Kinetics
  • Liver / enzymology
  • Lung / enzymology
  • Mixed Function Oxygenases / genetics*
  • Mixed Function Oxygenases / metabolism
  • Molecular Sequence Data
  • RNA / genetics
  • RNA / isolation & purification
  • Recombinant Proteins / metabolism
  • Substrate Specificity

Substances

  • Recombinant Proteins
  • RNA
  • DNA
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • Cytochrome P-450 CYP4A

Associated data

  • GENBANK/L04613
  • GENBANK/L04614
  • GENBANK/L04615
  • GENBANK/L04616
  • GENBANK/L04630
  • GENBANK/L04631
  • GENBANK/L04632
  • GENBANK/L04633
  • GENBANK/L04634
  • GENBANK/L04751