Molecular cloning and functional expression of rat cDNAs encoding the receptor for pituitary adenylate cyclase activating polypeptide (PACAP)

Biochem Biophys Res Commun. 1993 Jul 15;194(1):133-43. doi: 10.1006/bbrc.1993.1795.

Abstract

Two types of cDNA encoding PACAP receptors were isolated from the rat brain cDNA library by homology screening with a cDNA probe for VIP receptor. Nucleotide sequence analysis indicated that these two types of receptor mRNA were generated by alternative splicing mechanisms. These two cloned cDNAs were introduced into CHO cells respectively. Resultant transformants showed specific binding to [125I]PACAP27 which was displaced by unlabeled PACAP27 but not by VIP. Thus, these receptors are two subtypes of Type I PACAP receptor (Type I-A and Type I-B). The amino acid sequences of rat PACAP receptors deduced by the cDNAs showed a remarkable similarity with rat receptors for VIP, secretin, glucagon, and GHRH. A 6.5 kb significant hybridizing signal of the PACAP receptor mRNA was detected in the rat brain, and slight signal was also detected in the lung and the liver.

Publication types

  • Comparative Study

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism*
  • Cloning, Molecular / methods
  • DNA
  • Gene Expression*
  • Gene Library
  • Male
  • Molecular Sequence Data
  • Organ Specificity
  • Pituitary Gland / metabolism*
  • Poly A / genetics
  • Poly A / isolation & purification
  • Protein Structure, Secondary
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Receptors, Gastrointestinal Hormone / genetics
  • Receptors, Gastrointestinal Hormone / metabolism
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
  • Receptors, Pituitary Hormone*
  • Receptors, Vasoactive Intestinal Peptide
  • Sequence Homology, Amino Acid
  • Vasoactive Intestinal Peptide / metabolism

Substances

  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Gastrointestinal Hormone
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
  • Receptors, Pituitary Hormone
  • Receptors, Vasoactive Intestinal Peptide
  • Poly A
  • Vasoactive Intestinal Peptide
  • RNA
  • DNA

Associated data

  • GENBANK/D14584
  • GENBANK/D14720
  • GENBANK/D14908
  • GENBANK/D14909
  • GENBANK/S63836
  • GENBANK/S63837
  • GENBANK/S63838
  • GENBANK/U11085
  • GENBANK/U11086
  • GENBANK/U11087