Abstract
The partially CD4-expressing T cell clone, Vpr-1, which carries a latent vpr-defective HIV-1 genome and expresses HIV-1 Nef protein only, was permissive to superinfection by HIV-1. Superinfection of Vpr-1 with vif- or vpu-defective mutants, which were noncytopathic, reactivated the vpr-defective virus and led to homologous recombination and cytopathogenesis. The data provide an experimental model for homologous recombination being an important mechanism whereby HIV-1 acquires genetic heterogeneity, and when occurring among defective virus in vivo bestows novel biological activities and virulence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / virology*
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Clone Cells
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Gene Expression Regulation, Viral
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Gene Products, nef / biosynthesis
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Gene Products, nef / genetics
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Gene Products, vif / biosynthesis
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Gene Products, vif / genetics
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Gene Products, vpr / biosynthesis
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Gene Products, vpr / genetics
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Genome, Viral
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HIV Infections / genetics*
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HIV-1 / genetics*
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HIV-1 / pathogenicity
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Humans
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Mutation
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Recombination, Genetic
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Superinfection / genetics*
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nef Gene Products, Human Immunodeficiency Virus
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vif Gene Products, Human Immunodeficiency Virus
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vpr Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, nef
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Gene Products, vif
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Gene Products, vpr
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nef Gene Products, Human Immunodeficiency Virus
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vif Gene Products, Human Immunodeficiency Virus
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vpr Gene Products, Human Immunodeficiency Virus