Superinfection of a defective human immunodeficiency virus type 1 provirus-carrying T cell clone with vif or vpu mutants gives cytopathic virus particles by homologous recombination

AIDS Res Hum Retroviruses. 1995 Jan;11(1):45-53. doi: 10.1089/aid.1995.11.45.

Abstract

The partially CD4-expressing T cell clone, Vpr-1, which carries a latent vpr-defective HIV-1 genome and expresses HIV-1 Nef protein only, was permissive to superinfection by HIV-1. Superinfection of Vpr-1 with vif- or vpu-defective mutants, which were noncytopathic, reactivated the vpr-defective virus and led to homologous recombination and cytopathogenesis. The data provide an experimental model for homologous recombination being an important mechanism whereby HIV-1 acquires genetic heterogeneity, and when occurring among defective virus in vivo bestows novel biological activities and virulence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / virology*
  • Clone Cells
  • Gene Expression Regulation, Viral
  • Gene Products, nef / biosynthesis
  • Gene Products, nef / genetics
  • Gene Products, vif / biosynthesis
  • Gene Products, vif / genetics
  • Gene Products, vpr / biosynthesis
  • Gene Products, vpr / genetics
  • Genome, Viral
  • HIV Infections / genetics*
  • HIV-1 / genetics*
  • HIV-1 / pathogenicity
  • Humans
  • Mutation
  • Recombination, Genetic
  • Superinfection / genetics*
  • nef Gene Products, Human Immunodeficiency Virus
  • vif Gene Products, Human Immunodeficiency Virus
  • vpr Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, nef
  • Gene Products, vif
  • Gene Products, vpr
  • nef Gene Products, Human Immunodeficiency Virus
  • vif Gene Products, Human Immunodeficiency Virus
  • vpr Gene Products, Human Immunodeficiency Virus