Cloning and sequencing of the gene coding for dextranase from Streptococcus salivarius

Y Ohnishi; S Kubo; Y Ono; M Nozaki; Y Gonda; H Okano; T Matsuya; A Matsushiro; T Morita

doi:10.1016/0378-1119(95)00071-d

Cloning and sequencing of the gene coding for dextranase from Streptococcus salivarius

Gene. 1995 Apr 14;156(1):93-6. doi: 10.1016/0378-1119(95)00071-d.

Authors

Y Ohnishi¹, S Kubo, Y Ono, M Nozaki, Y Gonda, H Okano, T Matsuya, A Matsushiro, T Morita

Affiliation

¹ Department of Microbial Genetics, Osaka University, Suita, Japan.

PMID: 7737522
DOI: 10.1016/0378-1119(95)00071-d

Abstract

We cloned and sequenced the dextranase (Dex) (1,6-alpha-glucanhydrolase; EC 3.2.1.11)-encoding gene from Streptococcus salivarius (Ss) strain M-33. Recombinant clones from an Ss genomic library specifying Dex activity were identified as colonies surrounded by transparent halos on blue dextran plates. One of the clones had a 4.3-kb KpnI fragment containing the gene coding for an 826-amino-acid polypeptide with a molecular mass of 87.9 kDa, which corresponds well to that of native Dex from the Ss culture supernatant. There was no sequence homology between the gene encoding Ss Dex and the gene encoding dextran glucosidase of S. mutans, or between their protein products.

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Dextranase / chemistry
Dextranase / genetics*
Genes, Bacterial / genetics*
Molecular Sequence Data
Molecular Weight
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Streptococcus / enzymology
Streptococcus / genetics*

Substances

Dextranase

Associated data

GENBANK/D29644