Abstract
We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbenoxolone
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Carbocyanines / metabolism
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Cell Communication / drug effects
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Cell Communication / physiology*
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Cell Line
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Cell Line, Transformed
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Connexin 43 / genetics
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Fluoresceins / metabolism
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Fluorescent Dyes / metabolism*
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Gap Junctions / metabolism*
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Glycyrrhetinic Acid / analogs & derivatives
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Glycyrrhetinic Acid / pharmacology
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Microscopy, Fluorescence
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Oncogene Protein pp60(v-src) / genetics
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Stereoisomerism
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Succinates / pharmacology
Substances
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Carbocyanines
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Connexin 43
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Fluoresceins
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Fluorescent Dyes
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Succinates
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3,3'-dioctadecylindocarbocyanine
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Oncogene Protein pp60(v-src)
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Carbenoxolone
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Glycyrrhetinic Acid
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fluorexon