PTF1 encodes an essential protein in Saccharomyces cerevisiae, which shows strong homology with a new putative family of PPIases

FEBS Lett. 1995 May 29;365(2-3):198-202. doi: 10.1016/0014-5793(95)00471-k.

Abstract

Complementation of a temperature sensitive mutant of the yeast Saccharomyces cerevisiae resulted in the isolation of PTF1 (processing/termination factor 1), an essential gene encoding a putative 3'-end processing or transcription termination factor of pre-mRNAs. Ptf1p shows significant homology to a newly discovered family of PPIases. This family is characterized by its insensitivity to immunosuppressive drugs and the lack of homology with cyclophilins and FK-506 binding proteins [Rahfeld et al. (1994) FEBS Lett. 352, 180-184]. Should Ptf1p display PPIase activity, it would be the first characterized, eukaryotic member of this putative family, which is essential for growth.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Isomerases / biosynthesis*
  • Amino Acid Isomerases / chemistry*
  • Amino Acid Isomerases / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / chemistry*
  • Carrier Proteins / genetics
  • DNA, Fungal / chemistry
  • DNA, Fungal / metabolism
  • Endodeoxyribonucleases / chemistry
  • Genes, Fungal*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Mutagenesis
  • NIMA-Interacting Peptidylprolyl Isomerase
  • Open Reading Frames
  • Peptidylprolyl Isomerase
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins
  • Sequence Deletion
  • Sequence Homology, Amino Acid
  • Temperature
  • Transcription Factors / biosynthesis*
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics

Substances

  • Carrier Proteins
  • DNA, Fungal
  • NIMA-Interacting Peptidylprolyl Isomerase
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Endodeoxyribonucleases
  • Amino Acid Isomerases
  • ESS1 protein, S cerevisiae
  • Peptidylprolyl Isomerase

Associated data

  • GENBANK/X85972