Abstract
The pho-5+ gene of Neurospora crassa, which encodes a high-affinity phosphate permease, has been cloned and analyzed. The deduced ORF of 1707 nucleotides is interrupted by a single 63-nt intron and codes for a protein of 569 amino acids (aa). This aa sequence has 48% identity with the high-affinity phosphate transporter of Saccharomyces cerevisiae, PHO84. The pho-5 null mutants have no obvious phenotype. Strains which contain a null mutation in pho-4, which encodes an additional high-affinity phosphate permease [Bowman et al., J. Bacteriol. 153 (1983) 292-296], also have no obvious phenotype. However, strains containing mutations in both pho-5 and pho-4 are unable to grow under phosphate-restrictive conditions.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Fungal / genetics
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Enzyme Induction
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal
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Genes, Fungal*
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Isoenzymes / genetics*
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Isoenzymes / metabolism
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / metabolism
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Molecular Sequence Data
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Mutation
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Neurospora crassa / enzymology
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Neurospora crassa / genetics*
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Neurospora crassa / growth & development
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Phosphate Transport Proteins*
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Phosphates / metabolism
Substances
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DNA, Fungal
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Fungal Proteins
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Isoenzymes
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Membrane Transport Proteins
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Phosphate Transport Proteins
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Phosphates
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phosphate permease