Abstract
We show here that ligation of surface immunoglobulin or CD40 receptors in conjunction with interleukin-4 induces the nuclear factor of activated T cells (NF-AT) in normal murine B cells, which is inhibited by cyclosporin (CsA). Lipopolysaccharide, which activates B cells by a Ca(2+)-independent, CsA-resistant pathway, does not induce NF-AT. The NF-AT complex in T cells and B cells appears to be identical, comprising both Fos and Jun proteins and the 120 kDa cytosolic component of NF-AT (NF-ATp). Our transfection experiments using a trimerized NF-AT site linked to the minimal IL-2 promoter driving luciferase activity demonstrate that NF-AT is functional in A20 B-lymphoma cells. These results therefore suggest that the induction of NF-AT forms part of the B cell response to both cross-linking antigens and T cell-generated signals.
MeSH terms
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Animals
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Antibodies, Anti-Idiotypic / pharmacology
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B-Lymphocytes / drug effects
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B-Lymphocytes / immunology*
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Base Sequence
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CD40 Ligand
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Cell Line
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Cross-Linking Reagents
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Cyclosporine / pharmacology
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DNA / genetics
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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Enhancer Elements, Genetic
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In Vitro Techniques
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Interleukin-2 / genetics
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Interleukin-4 / pharmacology
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Male
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Molecular Sequence Data
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NFATC Transcription Factors
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Nuclear Proteins*
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Proto-Oncogene Proteins c-fos / metabolism
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Proto-Oncogene Proteins c-jun / metabolism
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T-Lymphocytes / immunology
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics
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Transfection
Substances
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Antibodies, Anti-Idiotypic
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Cross-Linking Reagents
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DNA-Binding Proteins
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Interleukin-2
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Membrane Glycoproteins
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NFATC Transcription Factors
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Nuclear Proteins
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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Transcription Factors
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CD40 Ligand
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Interleukin-4
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Cyclosporine
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DNA