Abstract
The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aminoglycosides*
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Anti-Bacterial Agents / pharmacology*
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Anti-Bacterial Agents / radiation effects*
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Blotting, Southern
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Cell Line
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Cell Survival / drug effects
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Coumarins
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DNA / drug effects*
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DNA, Ribosomal / drug effects
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DNA-Binding Proteins / drug effects*
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Fanconi Anemia
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Genes / drug effects*
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Glycosides
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Humans
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Intercalating Agents / pharmacology*
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Methoxsalen / pharmacology
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Photochemistry
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Photosensitizing Agents / pharmacology*
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RNA, Ribosomal / genetics*
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Tetrahydrofolate Dehydrogenase / genetics*
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Xeroderma Pigmentosum
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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Coumarins
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DNA, Ribosomal
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DNA-Binding Proteins
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Glycosides
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Intercalating Agents
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Photosensitizing Agents
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RNA, Ribosomal
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gilvocarcin V
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DNA
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Tetrahydrofolate Dehydrogenase
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Methoxsalen