Molecular cloning of a novel human cDNA homologous to CDC10 in Saccharomyces cerevisiae

Biochem Biophys Res Commun. 1994 Jul 15;202(1):82-7. doi: 10.1006/bbrc.1994.1896.

Abstract

We isolated a novel human cDNA, termed hCDC10, whose predicted product showed a high degree of homology to the CDC10 protein of Saccharomyces cerevisiae. This cDNA contained an open reading frame of 1254 nucleotides encoding 418 amino acids, which included a GTP-binding motif, GX4GKS--DX2G--KXD. The predicted peptide sequence also revealed partial amino-acid identity (40-50%) with Diff 6 in Drosophila and with H5 in mouse. Each of these sequence homologues, including Saccharomyces cerevisiae CDC10, contains the GTP-binding motif. Northern blot analyses indicated that the hCDC10 gene is expressed ubiquitously in normal tissues.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain / metabolism
  • Cell Cycle Proteins*
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Drosophila / metabolism
  • Female
  • Fetus
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • GTP Phosphohydrolases
  • GTP-Binding Proteins / chemistry*
  • Humans
  • Male
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Septins
  • Sequence Homology, Amino Acid
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics*

Substances

  • Cell Cycle Proteins
  • DNA, Complementary
  • Fungal Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • cdc10 protein, S pombe
  • CDC10 protein, S cerevisiae
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • Sept7 protein, mouse
  • Septins

Associated data

  • GENBANK/S72008