Abstract
We isolated a novel human cDNA, termed hCDC10, whose predicted product showed a high degree of homology to the CDC10 protein of Saccharomyces cerevisiae. This cDNA contained an open reading frame of 1254 nucleotides encoding 418 amino acids, which included a GTP-binding motif, GX4GKS--DX2G--KXD. The predicted peptide sequence also revealed partial amino-acid identity (40-50%) with Diff 6 in Drosophila and with H5 in mouse. Each of these sequence homologues, including Saccharomyces cerevisiae CDC10, contains the GTP-binding motif. Northern blot analyses indicated that the hCDC10 gene is expressed ubiquitously in normal tissues.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Blotting, Northern
-
Brain / metabolism
-
Cell Cycle Proteins*
-
Cloning, Molecular
-
DNA, Complementary / metabolism
-
Drosophila / metabolism
-
Female
-
Fetus
-
Fungal Proteins / chemistry
-
Fungal Proteins / genetics*
-
GTP Phosphohydrolases
-
GTP-Binding Proteins / chemistry*
-
Humans
-
Male
-
Membrane Proteins
-
Mice
-
Molecular Sequence Data
-
Organ Specificity
-
RNA, Messenger / metabolism
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism*
-
Saccharomyces cerevisiae Proteins
-
Schizosaccharomyces pombe Proteins
-
Septins
-
Sequence Homology, Amino Acid
-
Transcription Factors / chemistry*
-
Transcription Factors / genetics*
Substances
-
Cell Cycle Proteins
-
DNA, Complementary
-
Fungal Proteins
-
Membrane Proteins
-
RNA, Messenger
-
Saccharomyces cerevisiae Proteins
-
Schizosaccharomyces pombe Proteins
-
Transcription Factors
-
cdc10 protein, S pombe
-
CDC10 protein, S cerevisiae
-
GTP Phosphohydrolases
-
GTP-Binding Proteins
-
Sept7 protein, mouse
-
Septins