Abstract
We have analyzed the phenotype of five human monocytoid cell lines (U-937, THP-1, RC.2A, Mono Mac 6, and DD) and their capacity to support replication of HIV-1 strains with known tropism for T lymphocytes and mononuclear phagocytes. HIV-1 Ba-L, a prototype monocytotropic HIV-1 strain, was unable to establish continuous replication in any of the cell lines used. In contrast, the lymphotropic strain IIIB could replicate in all CD4-positive cell lines. We conclude that the capacity of HIV-1 isolates to replicate in established human monocytoid malignant cells does not correlate with the tropism of the virus for primary mononuclear phagocytes. Since monocytoid cell lines do not distinguish HIV-1 variants tropic for mononuclear phagocytes, we suggest the use of primary cells for studies of HIV-1 tropism.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acquired Immunodeficiency Syndrome / pathology
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Acquired Immunodeficiency Syndrome / physiopathology
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Cell Line
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Cell Movement / physiology
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Cytokines / metabolism
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Fluorescent Antibody Technique
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HIV-1 / isolation & purification
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HIV-1 / physiology*
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Humans
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In Vitro Techniques
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Leukemia, Monocytic, Acute / pathology
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Leukemia, Myelomonocytic, Acute / pathology
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Lymphocytes / cytology
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Lymphocytes / physiology
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Lymphoma, Large B-Cell, Diffuse / pathology
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Monocytes / cytology
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Monocytes / microbiology
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Monocytes / physiology*
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Phagocytes / cytology
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Phagocytes / microbiology
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Phagocytes / physiology
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Phenotype
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T-Lymphocytes / cytology
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T-Lymphocytes / microbiology
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T-Lymphocytes / physiology
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Tumor Cells, Cultured
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Virus Replication