Abstract
We show here that the state of the p34cdc2-p56cdc13 mitotic B cyclin complex determines whether a fission yeast cell undergoes S phase or mitosis. Mutants defective for p56cdc13 reset to G1 and rereplicate their DNA, while cells completely lacking the p34cdc2-p56cdc13 complex undergo multiple rounds of S phase. In contrast, formation of the p34cdc2-p56cdc13 complex in G1 promotes cells inappropriately into mitosis. We propose that the temporal order of S phase and mitosis is maintained by the presence or absence of the p34cdc2-p56cdc13 complex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CDC2 Protein Kinase / genetics
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CDC2 Protein Kinase / metabolism*
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Cell Compartmentation
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Cyclin B
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Cyclins / genetics
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Cyclins / metabolism*
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DNA Replication / physiology
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G1 Phase / physiology
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Mitosis / genetics
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Mitosis / physiology*
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Models, Biological
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Mutation
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Protein Binding
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Recombinant Proteins / metabolism
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S Phase / genetics
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S Phase / physiology*
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Schizosaccharomyces / genetics
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Schizosaccharomyces / growth & development*
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Time Factors
Substances
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Cyclin B
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Cyclins
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Recombinant Proteins
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CDC2 Protein Kinase