Abstract
Taxomyces andreanae, a fungal endophyte, was isolated from the phloem (inner bark) of the Pacific yew, Taxus brevifolia. The fungus is hyphomyceteous and, when grown in a semi-synthetic liquid medium, produced taxol and related compounds. Taxol was identified by mass spectrometry, chromatography, and reactivity with monoclonal antibodies specific for taxol. Both [1-14C]acetic acid and L-[U-14C]phenylalanine served as precursors of [14C]taxol in fungal cultures. No taxol was detected in zero-time cultures or in the small agar plugs used to inoculate the culture flasks.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Comment
MeSH terms
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Antibodies, Monoclonal
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Bridged Bicyclo Compounds / metabolism*
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Bridged-Ring Compounds*
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Chromatography, Thin Layer
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Culture Media
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Humans
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Immunoenzyme Techniques
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KB Cells
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Mitosporic Fungi / metabolism*
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Paclitaxel / analysis
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Paclitaxel / biosynthesis*
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Paclitaxel / chemistry
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Paclitaxel / pharmacology
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Spectrometry, Mass, Fast Atom Bombardment
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Taxoids*
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Trees / microbiology*
Substances
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Antibodies, Monoclonal
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Bridged Bicyclo Compounds
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Bridged-Ring Compounds
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Culture Media
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Taxoids
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taxane
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Paclitaxel