Is the IFN-gamma-induced enhancement of superoxide production in CGD-phagocytes caused by increased expression of the p47-phox cytosolic protein

Immunodeficiency. 1993;4(1-4):187-90.

Abstract

Interferon-gamma (IFN-gamma) had been shown to increase superoxide production of cultured monocytes from patients with chronic granulomatous disease (CGD). To elucidate the mechanism of the IFN-gamma-induced improvement of superoxide production of cultured monocytes from patients with CGD we examined the influence of IFN-gamma on the expression and the activity of the NADPH-oxidase in the monocytic cell-line Mono Mac 6. After cultivation of Mono Mac 6-cells in the presence of 500 U/ml IFN-gamma the superoxide production was found to be increased as well as the expression of the p47-phox cytosolic protein of the phagocytic NADPH-oxidase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cytosol / metabolism
  • Genes, Recessive
  • Granulomatous Disease, Chronic / genetics
  • Granulomatous Disease, Chronic / immunology
  • Granulomatous Disease, Chronic / metabolism*
  • Humans
  • Interferon-gamma / pharmacology*
  • Monocytes / drug effects
  • Monocytes / immunology
  • Monocytes / metabolism
  • NADPH Dehydrogenase / deficiency
  • NADPH Dehydrogenase / metabolism*
  • NADPH Oxidases
  • Phagocytes / drug effects
  • Phagocytes / immunology
  • Phagocytes / metabolism*
  • Phosphoproteins / deficiency
  • Phosphoproteins / metabolism*
  • Superoxides / metabolism

Substances

  • Phosphoproteins
  • Superoxides
  • Interferon-gamma
  • NADPH Oxidases
  • neutrophil cytosolic factor 1
  • NADPH Dehydrogenase