Abstract
In this study we demonstrate that adherence of peripheral blood mononuclear cells (PBMC) to either plastic or extracellular matrix, such as collagen type I or fibronectin, is a significant stimulus for the induction of both interleukin-8 (IL-8) mRNA and antigen. In addition, adherence of PBMC in the presence of lipopolysaccharide, interleukin-1-beta, or tumor necrosis factor-alpha greatly enhances transcription/translation of IL-8 from these leukocytes. Our findings demonstrate that PBMC adherent to either plastic or physiological surfaces in combination with an inflammatory agonist is both a potent and efficacious signal for the expression and production of the neutrophil chemotactic/activating cytokine, IL-8.
MeSH terms
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Base Sequence
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Blotting, Northern
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Cell Adhesion*
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Collagen / pharmacology
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DNA, Complementary
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Dose-Response Relationship, Drug
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Enzyme-Linked Immunosorbent Assay
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Extracellular Matrix / physiology
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Fibronectins / pharmacology
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Humans
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In Vitro Techniques
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Interleukin-1 / pharmacology*
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Interleukin-8 / biosynthesis*
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Interleukin-8 / blood
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Kinetics
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / drug effects
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Leukocytes, Mononuclear / metabolism*
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Lipopolysaccharides / pharmacology*
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Molecular Sequence Data
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Oligonucleotide Probes
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RNA, Messenger / biosynthesis
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RNA, Messenger / blood
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Recombinant Proteins / pharmacology
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Tumor Necrosis Factor-alpha / pharmacology*
Substances
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DNA, Complementary
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Fibronectins
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Interleukin-1
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Interleukin-8
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Lipopolysaccharides
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Oligonucleotide Probes
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RNA, Messenger
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Recombinant Proteins
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Tumor Necrosis Factor-alpha
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Collagen