Cloning the RAD51 homologue of Schizosaccharomyces pombe

Nucleic Acids Res. 1993 Sep 25;21(19):4586-91. doi: 10.1093/nar/21.19.4586.

Abstract

The RAD51 gene of Saccharomyces cerevisiae encodes a RecA like protein, which is involved in the recombinational repair of double strand breaks. We have isolated the RAD51 homologue, rhp51+, of the distantly related yeast strain Schizosaccharomyces pombe by heterologous hybridization. DNA sequence analysis of the rhp51+ gene revealed an open reading frame of 365 amino acids. Comparison of the amino acid sequences of RAD51 and rhp51+ showed a high level of conservation: 69% identical amino acids. There are two Mlul sites in the upstream region which may be associated with cell cycle regulation of the rhp51+ gene. The rhp51+ null allele, constructed by disruption of the coding region, is extremely sensitive to X-rays, indicating that the rhp51+ gene, like RAD51, is also involved in the repair of X-ray damage. The structural and functional homology between rhp51+ and RAD51 suggests evolutionary conservation of certain steps in the recombinational repair pathway.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA Repair*
  • DNA-Binding Proteins / genetics*
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Rad51 Recombinase
  • Recombination, Genetic*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Fungal Proteins
  • RHP51 protein, S pombe
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • RAD51 protein, S cerevisiae
  • Rad51 Recombinase

Associated data

  • GENBANK/Z22691