Abstract
We have devised a complementation assay in yeast to clone mammalian transcriptional activators and have used it to identify a human basic leucine-zipper transcription factor that we have designated Nrf1 for NF-E2-related factor 1. Nrf1 potentially encodes a 742-aa protein and displays marked homology to the mouse and human NF-E2 transcription factors. Nrf1 activates transcription via NF-E2 binding sites in yeast cells. The ubiquitous expression pattern of Nrf1 and the range of promoters containing the NF-E2 binding motif suggest that this gene may play a role in the regulation of heme synthesis and ferritin genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Complementary / genetics
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DNA-Binding Proteins / genetics*
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Erythropoiesis
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Gene Expression
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Genetic Complementation Test
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Humans
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Leucine Zippers
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Molecular Sequence Data
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NF-E2-Related Factor 1
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Nuclear Respiratory Factor 1
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Nuclear Respiratory Factors
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Oligodeoxyribonucleotides / chemistry
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RNA, Messenger / genetics
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Saccharomyces cerevisiae
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Sequence Alignment
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Sequence Homology, Amino Acid
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Tissue Distribution
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Trans-Activators*
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Transcription Factors / genetics*
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Transcription, Genetic
Substances
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DNA, Complementary
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DNA-Binding Proteins
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NF-E2-Related Factor 1
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NRF1 protein, human
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Nuclear Respiratory Factor 1
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Nuclear Respiratory Factors
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Oligodeoxyribonucleotides
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RNA, Messenger
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Trans-Activators
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Transcription Factors