Cloning of Nrf1, an NF-E2-related transcription factor, by genetic selection in yeast

Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11371-5. doi: 10.1073/pnas.90.23.11371.

Abstract

We have devised a complementation assay in yeast to clone mammalian transcriptional activators and have used it to identify a human basic leucine-zipper transcription factor that we have designated Nrf1 for NF-E2-related factor 1. Nrf1 potentially encodes a 742-aa protein and displays marked homology to the mouse and human NF-E2 transcription factors. Nrf1 activates transcription via NF-E2 binding sites in yeast cells. The ubiquitous expression pattern of Nrf1 and the range of promoters containing the NF-E2 binding motif suggest that this gene may play a role in the regulation of heme synthesis and ferritin genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics*
  • Erythropoiesis
  • Gene Expression
  • Genetic Complementation Test
  • Humans
  • Leucine Zippers
  • Molecular Sequence Data
  • NF-E2-Related Factor 1
  • Nuclear Respiratory Factor 1
  • Nuclear Respiratory Factors
  • Oligodeoxyribonucleotides / chemistry
  • RNA, Messenger / genetics
  • Saccharomyces cerevisiae
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Trans-Activators*
  • Transcription Factors / genetics*
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • NF-E2-Related Factor 1
  • NRF1 protein, human
  • Nuclear Respiratory Factor 1
  • Nuclear Respiratory Factors
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Trans-Activators
  • Transcription Factors

Associated data

  • GENBANK/L24123