Abstract
A two-hybrid protein interaction screen was used to isolate cDNAs encoding human proteins that can interact with human CDK2 in yeast. A new member of the retinoblastoma susceptibility gene family, Rbr-2 (Rb-related), was obtained. The sequence of the Rbr-2 protein shares approximately 50% identify with p107 and homology to Rb within the pocket domain. Several lines of evidence indicate that Rbr-2 is the adenovirus E1A-associated p130. Like Rb and p107, p130Rbr-2 can bind to viral oncoproteins, SV40 large T antigen, and adenovirus E1A through its pocket domain. Although p130Rbr-2 does not bind to CDK2 in vitro, it can interact with cyclins, with a clear preference for D-type cyclins. Because both CDK2 and p130Rbr-2 show affinity for cyclins, we suggest that p130Rbr-2 and CDK2 interacted through a yeast-derived cyclin bridge in the two-hybrid screen. The gene encoding p130Rbr-2 mapped to 16q13, a region of frequent genomic alteration in human tumors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenovirus E1A Proteins / metabolism
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Amino Acid Sequence
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Antigens, Polyomavirus Transforming / metabolism
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Base Sequence
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CDC2-CDC28 Kinases*
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Chromosome Mapping
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinases*
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Cyclins / metabolism*
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Humans
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Molecular Sequence Data
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Phosphoproteins*
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Protein Binding
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Protein Kinases / metabolism*
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Protein Serine-Threonine Kinases*
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Proteins / chemistry
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Proteins / genetics*
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Proteins / metabolism
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Retinoblastoma-Like Protein p130
Substances
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Adenovirus E1A Proteins
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Antigens, Polyomavirus Transforming
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Cyclins
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Phosphoproteins
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Proteins
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RBL2 protein, human
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Retinoblastoma-Like Protein p130
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Protein Kinases
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Protein Serine-Threonine Kinases
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CDC2-CDC28 Kinases
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CDK2 protein, human
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinases
Associated data
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GENBANK/L11593
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GENBANK/L11594
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GENBANK/L16016
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GENBANK/L24529
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GENBANK/S56762
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GENBANK/S56763
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GENBANK/S66936
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GENBANK/S67171
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GENBANK/Z15047
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GENBANK/Z15048