Abstract
The key cell-cycle regulator Cdc2 belongs to a family of cyclin-dependent kinases in higher eukaryotes. Dominant-negative mutations were used to address the requirement for kinases of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant arrested cells at the G2 to M phase transition, whereas mutants of the cyclin-dependent kinases Cdk2 and Cdk3 caused a G1 block. The mutant phenotypes were specifically rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to Cdc2 and Cdk2, executes a distinct and essential function in the mammalian cell cycle.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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CDC2 Protein Kinase / physiology
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CDC2-CDC28 Kinases*
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Cell Cycle / physiology*
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinases*
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Cyclins / physiology*
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Genetic Vectors
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Humans
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Molecular Sequence Data
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Mutation
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Plasmids
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Protein Kinases / genetics
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Protein Kinases / physiology*
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Protein Serine-Threonine Kinases*
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Tumor Cells, Cultured
Substances
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Cyclins
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Protein Kinases
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Protein Serine-Threonine Kinases
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CDC2 Protein Kinase
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CDC2-CDC28 Kinases
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CDK2 protein, human
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinases