Abstract
A negatively photo-regulated gene (bcb) has been isolated by means of differential hybridization of a genomic library of Arabidopsis thaliana. In mature plants, a 20-fold increase in the amount of steady-state bcb mRNA can be detected upon 48 h of dark adaption. The expression level of the gene is also dependent upon the developmental stage of the plant. The 21.5-kDa gene product (BCB) shows extensive similarity with blue Cu(2+)-binding proteins such as plastocyanin and stellacyanin. The protein abundance increases only twofold upon dark adaption, which implies the presence of post-transcriptional control. The isolation of a novel negatively photoregulated gene allows us to investigate the complex expression profile of genes responding to the absence of light.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics*
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Arabidopsis Proteins*
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Azurin / analogs & derivatives*
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Azurin / genetics
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Base Sequence
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Carrier Proteins / genetics*
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Copper*
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DNA, Complementary
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Gene Expression Regulation* / radiation effects
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Light*
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Membrane Proteins / genetics
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Molecular Sequence Data
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Plant Proteins / genetics*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Arabidopsis Proteins
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BCB protein, Arabidopsis
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Carrier Proteins
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DNA, Complementary
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Membrane Proteins
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Plant Proteins
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copper-binding protein
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cupredoxin
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Azurin
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Copper
Associated data
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GENBANK/L11589
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GENBANK/L11590
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GENBANK/L11591
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GENBANK/L11592
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GENBANK/L11593
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GENBANK/L11594
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GENBANK/L24529
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GENBANK/Z15047
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GENBANK/Z15048
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GENBANK/Z15058