Paralysis and early death in cysteine string protein mutants of Drosophila

Science. 1994 Feb 18;263(5149):977-80. doi: 10.1126/science.8310297.

Abstract

Multimeric complexes of synaptic vesicle and terminal membrane proteins are important components of the neurotransmitter release mechanism. The csp gene of Drosophila encodes proteins homologous to synaptic vesicle proteins in Torpedo. Monoclonal antibodies demonstrate different distributions of isoforms at distinct subsets of terminals. Deletion of the csp gene in Drosophila causes a temperature-sensitive block of synaptic transmission, followed by paralysis and premature death.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / physiology*
  • Electroretinography
  • Gene Deletion
  • Genes, Insect*
  • Genes, Lethal
  • Genetic Complementation Test
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Mutagenesis, Site-Directed
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / physiology
  • Phenotype
  • Photoreceptor Cells, Invertebrate / physiology*
  • Presynaptic Terminals / chemistry
  • Presynaptic Terminals / physiology*
  • Synaptic Transmission*

Substances

  • Membrane Proteins
  • Nerve Tissue Proteins