Abstract
Genes tbp1 and tbp2, encoding the transferrin-binding proteins Tbp1 and Tbp2, have been isolated from two strains of Neisseria meningitidis. The tbp2 and tbp1 open reading frames are tandemly arranged in the genome with an 87-bp intergenic region, and the DNA region upstream from the tbp2-coding sequence contains domains homologous to Escherichia coli promoter consensus motives. Nucleotide sequence analysis suggests the existence of a Tbp1 precursor carrying an N-terminal signal peptide with a peptidase I cleavage site and of a Tbp2 precursor with N-terminal homology to lipoproteins, including a peptidase II cleavage site. Comparison of the Tbp1 deduced amino acid (aa) sequences from both strains showed about 76% aa homology, while those of Tbp2 revealed only about 47% aa homology. These comparisons should be extended to other Neisseria strains in order to evaluate further this genetic divergence further.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, Bacterial / genetics
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Base Sequence
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / immunology
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Cloning, Molecular
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Conserved Sequence
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Genes, Bacterial*
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Gram-Negative Bacteria / genetics
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Iron-Binding Proteins
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Lipoproteins / genetics
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Molecular Sequence Data
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Neisseria meningitidis / genetics*
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Open Reading Frames
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Protein Sorting Signals / genetics
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Receptors, Transferrin / genetics*
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Restriction Mapping
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Sequence Alignment
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Transferrin / metabolism
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Transferrin-Binding Proteins
Substances
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Antigens, Bacterial
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Carrier Proteins
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Iron-Binding Proteins
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Lipoproteins
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Protein Sorting Signals
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Receptors, Transferrin
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Transferrin
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Transferrin-Binding Proteins
Associated data
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GENBANK/L03653
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GENBANK/L03654
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GENBANK/L07632
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GENBANK/M96932
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GENBANK/M96933
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GENBANK/S65693
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GENBANK/S65694
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GENBANK/X54209
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GENBANK/Z15129
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GENBANK/Z15130
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GENBANK/Z35133