Human PCK1 encoding phosphoenolpyruvate carboxykinase is located on chromosome 20q13.2

Genomics. 1993 Jan;15(1):219-21. doi: 10.1006/geno.1993.1040.

Abstract

Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP) (PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK cDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Fungal
  • Chromosomes, Human, Pair 20*
  • Cricetinae
  • DNA, Single-Stranded
  • Genome, Human
  • Genomic Library
  • Humans
  • Hybrid Cells
  • Mice
  • Molecular Sequence Data
  • Phosphoenolpyruvate Carboxykinase (GTP) / genetics*
  • Rodentia

Substances

  • DNA, Single-Stranded
  • Phosphoenolpyruvate Carboxykinase (GTP)

Associated data

  • GENBANK/L01126
  • GENBANK/L01127
  • GENBANK/L01128
  • GENBANK/L01129
  • GENBANK/L01130
  • GENBANK/L01131
  • GENBANK/L12760
  • GENBANK/L20583
  • GENBANK/L20584
  • GENBANK/X68166