Abstract
The cDNA encoding adenovirus E1A enhancer-binding protein E1A-F was isolated by screening a HeLa cell lambda gt11 expression library for E1A-F site-specific DNA binding. One cDNA clone produced recombinant E1A-F protein with the same DNA binding specificity as that endogenous to HeLa cells. Sequence analysis of the cDNA showed homology with the ETS-domain, a region required for sequence-specific DNA binding and common to all ets oncogene members. Analysis of the longest cDNA revealed about a 94% identity in amino acids between human E1A-F and mouse PEA3 (polyomavirus enhancer activator 3), a recently characterized ets oncogene member. E1A-F was encoded by a 2.5kb mRNA in HeLa cells, which was found to increase during the early period of adenovirus infection. In contrast, ets-2 mRNA was significantly reduced in infected HeLa cells. The results indicate that E1A enhancer binding protein E1A-F is a member of the ets oncogene family and is probably a human homologue of mouse PEA3.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenovirus E1A Proteins / genetics*
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Adenovirus E1A Proteins / isolation & purification
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Adenoviruses, Human / genetics*
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Adenoviruses, Human / physiology
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cell Line
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DNA / isolation & purification
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / isolation & purification
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Enhancer Elements, Genetic*
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HeLa Cells
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Humans
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Methylation
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Mice
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Molecular Sequence Data
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Multigene Family
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Oncogenes*
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / isolation & purification
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Proto-Oncogene Proteins c-ets
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Recombinant Fusion Proteins / metabolism
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Retroviridae Proteins, Oncogenic / genetics*
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Retroviridae Proteins, Oncogenic / isolation & purification
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Sequence Homology, Amino Acid
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Transcription, Genetic
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beta-Galactosidase / metabolism
Substances
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Adenovirus E1A Proteins
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DNA-Binding Proteins
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ETV4 protein, human
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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Recombinant Fusion Proteins
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Retroviridae Proteins, Oncogenic
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oncogene proteins v-ets
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DNA
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beta-Galactosidase