Abstract
By in vitro transcription/translation of model proteins in the presence of dog pancreas microsomes, we have measured the minimum distance of an acceptor site from the lumenal end of a transmembrane segment required for N-linked glycosylation, both when the acceptor site is placed N- and C-terminally to the membrane anchor. We observe a sharp threshold at a distance of 12-14 residues, suggesting that the oligosaccharyltransferase active site is 30-40 A above the membrane and is oriented roughly parallel to the membrane surface.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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DNA
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Dogs
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Endopeptidases / metabolism
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Endoplasmic Reticulum / metabolism*
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Escherichia coli / enzymology
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Glycosylation
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Hexosyltransferases*
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Intracellular Membranes / metabolism
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Membrane Proteins*
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Microsomes / enzymology
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Molecular Sequence Data
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Pancreas / enzymology
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Protein Conformation
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Serine Endopeptidases*
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Transferases / chemistry
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Transferases / metabolism*
Substances
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Membrane Proteins
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DNA
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Transferases
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Hexosyltransferases
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dolichyl-diphosphooligosaccharide - protein glycotransferase
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Endopeptidases
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Serine Endopeptidases
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type I signal peptidase