Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp

Appl Environ Microbiol. 1995 Nov;61(11):4110-3. doi: 10.1128/aem.61.11.4110-4113.1995.

Abstract

A gene expressing xylanase activity was isolated from a genomic library of Thermotoga sp. strain FjSS3-B.1. The sequence of the gene shows that it encodes a single domain, family 10 xylanase. The recombinant enzyme has extremely high thermal stability, activity over a relatively broad pH range, and activity on Pinus radiata kraft pulp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biotechnology
  • DNA, Bacterial / genetics
  • Enzyme Stability
  • Gene Expression
  • Genes, Bacterial*
  • Gram-Negative Anaerobic Bacteria / enzymology*
  • Gram-Negative Anaerobic Bacteria / genetics*
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Paper
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Temperature
  • Xylan Endo-1,3-beta-Xylosidase
  • Xylosidases / genetics*
  • Xylosidases / metabolism*

Substances

  • DNA, Bacterial
  • Recombinant Proteins
  • Xylosidases
  • Xylan Endo-1,3-beta-Xylosidase

Associated data

  • GENBANK/U33060