Abstract
A gene expressing xylanase activity was isolated from a genomic library of Thermotoga sp. strain FjSS3-B.1. The sequence of the gene shows that it encodes a single domain, family 10 xylanase. The recombinant enzyme has extremely high thermal stability, activity over a relatively broad pH range, and activity on Pinus radiata kraft pulp.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Biotechnology
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DNA, Bacterial / genetics
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Enzyme Stability
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Gene Expression
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Genes, Bacterial*
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Gram-Negative Anaerobic Bacteria / enzymology*
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Gram-Negative Anaerobic Bacteria / genetics*
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Hydrogen-Ion Concentration
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Molecular Sequence Data
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Paper
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Temperature
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Xylan Endo-1,3-beta-Xylosidase
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Xylosidases / genetics*
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Xylosidases / metabolism*
Substances
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DNA, Bacterial
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Recombinant Proteins
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Xylosidases
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Xylan Endo-1,3-beta-Xylosidase