Abstract
Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;1 and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea. The second gene, A.h.Chi2;1, was only expressed after treatment with the fungal spores. Transgenic tobacco plants containing the complete peanut A.h.Chi2;1 gene exhibited essentially the same expression pattern in leaves as observed in peanut cell cultures. Expression characteristics of transgenic tobacco carrying a promoter-GUS fusion of A.h.Chi2;1 are described.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arachis / enzymology
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Arachis / genetics*
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Arachis / microbiology
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Base Sequence
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Chitinases / biosynthesis
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Chitinases / classification
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Chitinases / genetics*
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Ethylenes / pharmacology
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Gene Expression Regulation, Plant*
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Genes, Plant*
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Genes, Reporter
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Genomic Library
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Mitosporic Fungi / pathogenicity
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Molecular Sequence Data
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Nicotiana / genetics
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Plant Growth Regulators / pharmacology
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Plant Proteins / biosynthesis
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Plant Proteins / classification
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Plant Proteins / genetics*
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Plants, Genetically Modified
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Plants, Toxic
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Recombinant Proteins / biosynthesis
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Salicylates / pharmacology
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Salicylic Acid
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Sequence Homology, Amino Acid
Substances
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Ethylenes
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Plant Growth Regulators
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Plant Proteins
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Recombinant Proteins
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Salicylates
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pathogenesis-related proteins, plant
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ethylene
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CHI2,2 protein, Arachis hypogaea
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Chitinases
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chi2-1 protein, Arachis hypogaea
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Salicylic Acid
Associated data
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GENBANK/X82329
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GENBANK/X82330