Abstract
Chlamydomonas mutants missing the central pair or radial spokes are paralyzed despite the fact that they have the full wild-type complement of functional dynein ATPases. We show here that these mutants can move under conditions of low ATP concentration, a combination of ATP and ADP, and a combination of ATP and ribose-modified ATP analogs. These conditions suggest an inhibitory role of ATP and that this inhibition can be relieved by ADP or analogs. The function of the central-pair/radial spoke complex may be to release this ATP inhibition in a controlled manner.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adenosine Diphosphate / analogs & derivatives
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Adenosine Diphosphate / metabolism
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Adenosine Diphosphate / pharmacology
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Adenosine Triphosphate / analogs & derivatives
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Adenosine Triphosphate / metabolism
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Adenosine Triphosphate / pharmacology
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Animals
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Cell Movement / physiology*
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Chlamydomonas / cytology*
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Chlamydomonas / genetics
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Chlamydomonas / ultrastructure
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Dyneins / genetics
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Dyneins / metabolism
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Flagella / chemistry
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Flagella / physiology*
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Microscopy, Video
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Mutation / physiology
Substances
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Adenosine Diphosphate
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Adenosine Triphosphate
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Dyneins