Abstract
The proto-oncogene product Vav plays a critical role in hematopoietic signal transduction. By using the yeast two-hybrid system, we identified a novel human protein, ENX-1, which interacts specifically with Vav both in vitro and in vivo. ENX-1 represents the human homolog of the Drosophila Enhancer of zeste gene, a member of the Polycomb group of genes, which are transcriptional regulators of homeobox gene expression. Interaction with ENX-1 suggests that Vav functions as an upstream element in the transcriptional regulation of homeobox genes, known to be important effectors in the hematopoietic system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding Sites
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Cell Cycle Proteins*
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Cloning, Molecular
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DNA, Complementary / genetics
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Drosophila / genetics
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Drosophila Proteins*
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Gene Expression Regulation
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Genes, Homeobox*
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Genes, Insect
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Hematopoiesis / genetics
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Humans
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In Vitro Techniques
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Molecular Sequence Data
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Nuclear Proteins*
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Polycomb Repressive Complex 2
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Protein Binding
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Proteins / genetics
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Proteins / isolation & purification
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Proteins / metabolism*
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / isolation & purification
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-vav
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Repressor Proteins*
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Saccharomyces cerevisiae / genetics
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Sequence Homology, Amino Acid
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Signal Transduction / genetics
Substances
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Cell Cycle Proteins
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DNA, Complementary
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Drosophila Proteins
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MAS1 protein, human
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Nuclear Proteins
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Proteins
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-vav
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Repressor Proteins
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VAV1 protein, human
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E(z) protein, Drosophila
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Polycomb Repressive Complex 2
Associated data
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GENBANK/U17552
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GENBANK/U17563
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GENBANK/U17564
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GENBANK/U18003
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GENBANK/U18007
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GENBANK/U52965