Abstract
We recently cloned one of spliced variant forms of rat leptin receptor (OB-R), which contains a short intracellular domain, and found obese-phenotype-linked nucleotide alteration in the extracellular domain of the cDNA from the Zucker (fa/fa) rat, which results in a glutamine269 to proline269 amino acid substitution. Reported herein are the cloning and sequencing of another spliced variant forms of rat OB-R cDNA with a long intracellular domain. Both forms of OB-R cDNA share the same extracellular domain. In the Zucker (fa/fa) rat, no changes in either the gene structure nor in the nucleotide sequence of the long intracellular domain were observed. However, the expression level of OB-R mRNA in the brain of Zucker (fa/fa) rat was higher than for lean littermates. These facts suggest that the substitution at codon 269 of the OB-R cDNA represents the crucial mutation which results in the obese phenotype of Zucker (fa/fa) rat.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Cloning, Molecular
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Codon
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DNA Primers
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DNA, Complementary
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Genetic Variation
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Glutamine*
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Humans
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Male
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Mice
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Molecular Sequence Data
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Obesity / genetics*
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Organ Specificity
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Point Mutation*
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Polymerase Chain Reaction
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Proline*
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RNA, Messenger / biosynthesis
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Rats
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Rats, Sprague-Dawley
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Rats, Wistar
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Rats, Zucker / genetics*
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Receptors, Cell Surface*
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Receptors, Leptin
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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Carrier Proteins
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Codon
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DNA Primers
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DNA, Complementary
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LEPR protein, human
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RNA, Messenger
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Receptors, Cell Surface
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Receptors, Leptin
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Recombinant Proteins
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leptin receptor, mouse
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Glutamine
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Proline
Associated data
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GENBANK/D84550
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GENBANK/D84551