Identification of an H2-M3-restricted Listeria epitope: implications for antigen presentation by M3

Immunity. 1996 Jul;5(1):63-72. doi: 10.1016/s1074-7613(00)80310-6.

Abstract

Using expression cloning, we have identified an H2-M3-restricted epitope of the intracellular bacterial pathogen Listeria monocytogenes. Picomolar concentrations of an amino-terminal N-formylated hexapeptide, fMIGWII, targeted cells for lysis by CD8+ cytotoxic T cells, while the nonformylated peptide was approximately 100-fold less active. The sequence of the 185 aa protein source of this epitope predicts a transmembrane protein that retains its N terminus and assumes an N(out)-C(in) topology. This membrane orientation offers an explanation for the protection of the epitope from deformylases present in the bacterial cell and suggests an explanation for the ability of phagocytes to present H2-M3-restricted bacterial epitopes via a vacuolar TAP-independent mechanism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Antigen Presentation* / genetics
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology
  • Antigens, Bacterial / isolation & purification*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology
  • Bacterial Proteins / isolation & purification*
  • Base Sequence
  • Cytotoxicity, Immunologic / genetics
  • Epitopes / genetics
  • Epitopes / immunology
  • Epitopes / isolation & purification*
  • Gene Library
  • H-2 Antigens / genetics
  • H-2 Antigens / immunology*
  • Listeria monocytogenes / genetics
  • Listeria monocytogenes / immunology*
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Membrane Proteins / isolation & purification
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • T-Lymphocytes, Cytotoxic / immunology
  • Transformation, Bacterial / immunology

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Epitopes
  • H-2 Antigens
  • LemA protein, Listeria
  • Membrane Proteins

Associated data

  • GENBANK/U66186