An improved method for the detection of peptide-induced upregulation of HLA-A2 molecules on TAP-deficient T2 cells

Exp Clin Immunogenet. 1996;13(1):30-5.

Abstract

Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP Binding Cassette Transporter, Subfamily B, Member 3
  • ATP-Binding Cassette Transporters / genetics*
  • Amino Acid Sequence / genetics
  • Cell Line
  • HLA-A2 Antigen / drug effects*
  • HLA-A2 Antigen / metabolism
  • Humans
  • Immunoassay / methods*
  • Major Histocompatibility Complex / genetics
  • Orthomyxoviridae / metabolism
  • Peptides / metabolism
  • Peptides / pharmacology*
  • Protein Binding / immunology
  • Tumor Suppressor Protein p53 / metabolism
  • Tumor Suppressor Protein p53 / pharmacology
  • Up-Regulation / drug effects*
  • Viral Matrix Proteins / metabolism
  • Viral Matrix Proteins / pharmacology

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 2
  • ATP Binding Cassette Transporter, Subfamily B, Member 3
  • ATP-Binding Cassette Transporters
  • HLA-A2 Antigen
  • Peptides
  • TAP1 protein, human
  • Tumor Suppressor Protein p53
  • Viral Matrix Proteins
  • TAP2 protein, human