Abstract
Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ATP Binding Cassette Transporter, Subfamily B, Member 2
-
ATP Binding Cassette Transporter, Subfamily B, Member 3
-
ATP-Binding Cassette Transporters / genetics*
-
Amino Acid Sequence / genetics
-
Cell Line
-
HLA-A2 Antigen / drug effects*
-
HLA-A2 Antigen / metabolism
-
Humans
-
Immunoassay / methods*
-
Major Histocompatibility Complex / genetics
-
Orthomyxoviridae / metabolism
-
Peptides / metabolism
-
Peptides / pharmacology*
-
Protein Binding / immunology
-
Tumor Suppressor Protein p53 / metabolism
-
Tumor Suppressor Protein p53 / pharmacology
-
Up-Regulation / drug effects*
-
Viral Matrix Proteins / metabolism
-
Viral Matrix Proteins / pharmacology
Substances
-
ATP Binding Cassette Transporter, Subfamily B, Member 2
-
ATP Binding Cassette Transporter, Subfamily B, Member 3
-
ATP-Binding Cassette Transporters
-
HLA-A2 Antigen
-
Peptides
-
TAP1 protein, human
-
Tumor Suppressor Protein p53
-
Viral Matrix Proteins
-
TAP2 protein, human