Abstract
Many of the antimicrobial, immunomodulatory and cell growth inhibitory activities of the interferons are mediated by interferon-inducible proteins. Earlier we characterized an interferon-inducible murine protein, p202, whose expression in transfected cells inhibits cell proliferation and which can form a complex with retinoblastoma protein (pRb). Here we report that in transfected cells expression of p202 inhibits E2F-stimulated transcription of a reporter gene and of endogenous genes. Inhibition of the transcriptional activity of E2F by p202 does not depend on fully functional pRb and is correlated with inhibition of the sequence-specific DNA binding of E2F. p202 interacts with the transcription factor E2F (E2F-1/DP-1) in vitro and in vivo. Inhibition of E2F activity by p202 may contribute to growth inhibition by the interferons.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carrier Proteins / pharmacology*
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Cell Cycle Proteins*
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Cell Division / drug effects
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DNA / metabolism
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DNA-Binding Proteins*
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E2F Transcription Factors
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E2F1 Transcription Factor
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Electrophoresis, Polyacrylamide Gel
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Female
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Genes, Reporter
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HeLa Cells
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Humans
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Interferons / physiology
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Intracellular Signaling Peptides and Proteins*
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Mice
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Nuclear Proteins / pharmacology*
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Phosphoproteins / pharmacology*
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Retinoblastoma-Binding Protein 1
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Transcription Factor DP1
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Transcription Factors / metabolism*
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Transcription, Genetic / drug effects*
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Tumor Cells, Cultured
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Tumor Suppressor p53-Binding Protein 1
Substances
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Arid4a protein, mouse
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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E2F Transcription Factors
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E2F1 Transcription Factor
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E2F1 protein, human
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E2f1 protein, mouse
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Ifi202b protein, mouse
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Intracellular Signaling Peptides and Proteins
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Nuclear Proteins
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Phosphoproteins
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Retinoblastoma-Binding Protein 1
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TP53BP1 protein, human
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Transcription Factor DP1
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Transcription Factors
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Tumor Suppressor p53-Binding Protein 1
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DNA
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Interferons