Inhibition of E2F-mediated transcription by p202

EMBO J. 1996 Oct 15;15(20):5668-78.

Abstract

Many of the antimicrobial, immunomodulatory and cell growth inhibitory activities of the interferons are mediated by interferon-inducible proteins. Earlier we characterized an interferon-inducible murine protein, p202, whose expression in transfected cells inhibits cell proliferation and which can form a complex with retinoblastoma protein (pRb). Here we report that in transfected cells expression of p202 inhibits E2F-stimulated transcription of a reporter gene and of endogenous genes. Inhibition of the transcriptional activity of E2F by p202 does not depend on fully functional pRb and is correlated with inhibition of the sequence-specific DNA binding of E2F. p202 interacts with the transcription factor E2F (E2F-1/DP-1) in vitro and in vivo. Inhibition of E2F activity by p202 may contribute to growth inhibition by the interferons.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carrier Proteins / pharmacology*
  • Cell Cycle Proteins*
  • Cell Division / drug effects
  • DNA / metabolism
  • DNA-Binding Proteins*
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Genes, Reporter
  • HeLa Cells
  • Humans
  • Interferons / physiology
  • Intracellular Signaling Peptides and Proteins*
  • Mice
  • Nuclear Proteins / pharmacology*
  • Phosphoproteins / pharmacology*
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors / metabolism*
  • Transcription, Genetic / drug effects*
  • Tumor Cells, Cultured
  • Tumor Suppressor p53-Binding Protein 1

Substances

  • Arid4a protein, mouse
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • E2F1 protein, human
  • E2f1 protein, mouse
  • Ifi202b protein, mouse
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • Phosphoproteins
  • Retinoblastoma-Binding Protein 1
  • TP53BP1 protein, human
  • Transcription Factor DP1
  • Transcription Factors
  • Tumor Suppressor p53-Binding Protein 1
  • DNA
  • Interferons