Abstract
A cDNA clone encoding the germ cell nuclear factor, GCNF, a member of the nuclear receptor superfamily has been isolated from the human embryonal carcinoma cell line NT2/D1. Sequencing of this clone reveals an open reading frame encoding a 476 amino acid protein. A comparison of the amino acid sequence of the human GCNF with its mouse homologue shows only six amino acid exchanges in the whole protein and a deletion in the amino-terminal region. Northern blot analysis demonstrates that the expression in the testis is conserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA-Binding Proteins / genetics*
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Gene Expression Regulation, Developmental / genetics
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Humans
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Molecular Sequence Data
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Nuclear Receptor Subfamily 6, Group A, Member 1
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Receptors, Cytoplasmic and Nuclear / genetics*
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Sequence Analysis
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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DNA-Binding Proteins
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NR6A1 protein, human
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Nr6a1 protein, mouse
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Nuclear Receptor Subfamily 6, Group A, Member 1
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Receptors, Cytoplasmic and Nuclear