Characterization of the human germ cell nuclear factor gene

Biochim Biophys Acta. 1996 Dec 11;1309(3):179-82. doi: 10.1016/s0167-4781(96)00157-1.

Abstract

A cDNA clone encoding the germ cell nuclear factor, GCNF, a member of the nuclear receptor superfamily has been isolated from the human embryonal carcinoma cell line NT2/D1. Sequencing of this clone reveals an open reading frame encoding a 476 amino acid protein. A comparison of the amino acid sequence of the human GCNF with its mouse homologue shows only six amino acid exchanges in the whole protein and a deletion in the amino-terminal region. Northern blot analysis demonstrates that the expression in the testis is conserved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation, Developmental / genetics
  • Humans
  • Molecular Sequence Data
  • Nuclear Receptor Subfamily 6, Group A, Member 1
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • NR6A1 protein, human
  • Nr6a1 protein, mouse
  • Nuclear Receptor Subfamily 6, Group A, Member 1
  • Receptors, Cytoplasmic and Nuclear

Associated data

  • GENBANK/S83309