Abstract
TSD2, a gene necessary for DNA synthesis in Ustilago maydis, was cloned by complementation of the temperature sensitive growth defect of a mutant known previously as pol1-1 and renamed here tsd2-1. Linkage analysis established that the cloned fragment contained an allele of tsd2-1 and not a suppressor. DNA sequence determination of the cloned DNA fragment indicated the presence of a single large uninterrupted open reading frame capable of encoding a protein of 845 amino acids without homology to any known gene involved in DNA synthesis. TSD2 was found to be cell cycle-regulated and mRNA levels peaked in early S or G1 phase.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics*
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Cloning, Molecular
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DNA Replication / genetics*
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DNA, Fungal / biosynthesis
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DNA, Fungal / genetics
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Fungal Proteins*
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G1 Phase
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Gene Expression Regulation, Fungal
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Genes, Fungal*
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Genetic Complementation Test
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Molecular Sequence Data
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Open Reading Frames
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RNA, Fungal / genetics
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RNA, Fungal / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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S Phase
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Sequence Analysis, DNA
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Ustilago / cytology
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Ustilago / genetics*
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Ustilago / metabolism
Substances
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Cell Cycle Proteins
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DNA, Fungal
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Fungal Proteins
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RNA, Fungal
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RNA, Messenger
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TSD2 protein, Ustilago maydis