Abstract
We examined the expression of the murine homologue of myotonic dystrophy associated homeodomain protein (mDMAHP) using two different strategies. The first approach, RT-PCR, detected spliced transcripts in a wide range of embryonic and adult tissues, in a pattern overlapping substantially with the expression of mDMPK. A second approach, the generation of transgenic mice expressing the lacZ reporter gene from a 4.3 kb promoter fragment, also demonstrated expression in a range of tissues with potential links to the phenotype in myotonic dystrophy. We conclude that murine DMAHP has a similar pattern of expression to human DMAHP and will serve as a useful model for functional studies of this gene, although species differences, such as the reduced CpG island (1.8 kb compared with 3.5 kb) must be carefully considered.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Age Factors
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
CpG Islands
-
Embryo, Mammalian / metabolism
-
Female
-
Gene Expression Regulation, Developmental*
-
Genes, Reporter
-
Homeodomain Proteins / genetics*
-
Homeodomain Proteins / metabolism*
-
Humans
-
Male
-
Mice
-
Mice, Inbred C57BL
-
Mice, Inbred CBA
-
Mice, Transgenic
-
Molecular Sequence Data
-
Myotonic Dystrophy / genetics*
-
Polymerase Chain Reaction
-
Pregnancy
-
RNA Splicing
-
RNA, Messenger / genetics
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Sequence Homology, Amino Acid
-
Transcription, Genetic
-
beta-Galactosidase / genetics
-
beta-Galactosidase / metabolism
Substances
-
Homeodomain Proteins
-
RNA, Messenger
-
Recombinant Fusion Proteins
-
SIX5 protein, human
-
Six5 protein, mouse
-
beta-Galactosidase