Abstract
This article describes the methods used for the purification of heat shock proteins gp96, hsp90, and hsp70 from murine and human tissues and cell lines. The heat shock proteins purified by the methods described are associated noncovalently with an array of cellular peptides. The use of heat shock protein-peptide complexes for eliciting prophylactic and therapeutic immunity to cancers and infectious diseases is discussed.
MeSH terms
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Animals
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Cancer Vaccines* / immunology
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Cancer Vaccines* / therapeutic use
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Cell Line
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Communicable Diseases / immunology*
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Communicable Diseases / therapy
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Heat-Shock Proteins / immunology*
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Heat-Shock Proteins / isolation & purification*
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Humans
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Mice
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Neoplasms / immunology*
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Neoplasms / prevention & control
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Neoplasms / therapy
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Peptides / immunology
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Peptides / isolation & purification*
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Rats
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Vaccines* / immunology
Substances
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Cancer Vaccines
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Heat-Shock Proteins
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Peptides
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Vaccines