Abstract
Polysaccharopeptide (PSP) isolated from the edible mushroom Coriolus versicolor was tested for its potential as an anti-human immunodeficiency virus type 1 (HIV-1) compound in a series of in vitro assays. It demonstrated inhibition of the interaction between HIV-1 gp 120 and immobilized CD4 receptor (IC50 = 150 microg/ml), potent inhibition of recombinant HIV-1 reverse transcriptase (IC50 = 6.25 microg/ml), and inhibited a glycohydrolase enzyme associated with viral glycosylation. These properties, coupled with its high solubility in water, heat-stability and low cytotoxicity, make it a useful compound for further studies on its possible use as an anti-viral agent in vivo.
MeSH terms
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Adjuvants, Immunologic / pharmacology
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Anti-HIV Agents / pharmacology*
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Antineoplastic Agents / pharmacology
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Basidiomycota
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CD4 Antigens / metabolism
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Glycoside Hydrolases / antagonists & inhibitors
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HIV Envelope Protein gp120 / metabolism
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HIV Infections / drug therapy*
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HIV Reverse Transcriptase / drug effects
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HIV-1 / drug effects*
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HIV-1 / metabolism
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Humans
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Immunoenzyme Techniques
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Proteoglycans / pharmacology*
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Reverse Transcriptase Inhibitors / pharmacology
Substances
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Adjuvants, Immunologic
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Anti-HIV Agents
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Antineoplastic Agents
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CD4 Antigens
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HIV Envelope Protein gp120
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Proteoglycans
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Reverse Transcriptase Inhibitors
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polysaccharide peptide
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HIV Reverse Transcriptase
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Glycoside Hydrolases