Abstract
PCTAIRE-1 is a member of the cyclin-dependent kinase (cdk)-like class of proteins, and is localized mainly in the mammalian brain. Using the yeast two-hybrid system we screened a mouse brain cDNA library with PCTAIRE-1 as bait, and isolated several clones coding for the mouse homologs of the following proteins: p11 (also known as calpactin I light chain) and the eta, theta (also known as tau) and zeta isoforms of 14-3-3 proteins. We confirmed that these four proteins interact with PCTAIRE-1 by demonstrating the biochemical interactions using the pure recombinant proteins. The fact that 14-3-3 proteins are known to interact with many other intracellular proteins (such as C-kinase, Raf, Bcr, P13-kinase) and p11 with annexin II (a major pp60(v-src) and C-kinase substrate) suggests that PCTAIRE-1 might be part of multiple signal transduction cascades and cellular protein networks.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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14-3-3 Proteins
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Amino Acid Sequence
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Animals
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Annexin A2 / genetics
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Annexin A2 / metabolism*
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Base Sequence
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Brain / enzymology
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Cloning, Molecular
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Cyclin-Dependent Kinases*
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Mice
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Molecular Sequence Data
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Molecular Weight
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Protein Binding
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Protein Serine-Threonine Kinases / chemistry
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Proteins / genetics
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Proteins / metabolism*
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Tyrosine 3-Monooxygenase*
Substances
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14-3-3 Proteins
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Annexin A2
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Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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Tyrosine 3-Monooxygenase
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Protein Serine-Threonine Kinases
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Cyclin-Dependent Kinases
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PCTAIRE-1 protein kinase
Associated data
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GENBANK/D83037
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GENBANK/U57311
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GENBANK/U57312
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GENBANK/U79231