Cloning and sequence analysis of the fimbriae associated protein (fap) gene from Actinobacillus actinomycetemcomitans

K Ishihara; K Honma; T Miura; T Kato; K Okuda

doi:10.1006/mpat.1997.0137

Cloning and sequence analysis of the fimbriae associated protein (fap) gene from Actinobacillus actinomycetemcomitans

Microb Pathog. 1997 Aug;23(2):63-9. doi: 10.1006/mpat.1997.0137.

Authors

K Ishihara¹, K Honma, T Miura, T Kato, K Okuda

Affiliation

¹ Department of Microbiology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba, 261, Japan.

PMID: 9245617
DOI: 10.1006/mpat.1997.0137

Abstract

Fimbrial-associated protein, an attachment factor of Actinobacillus actinomycetemcomitans, was genetically analysed by cloning. The plasmid obtained was found to harbor a 1.7kb fragment which contained a 228bp open reading frame encoding 76 amino acids (7.970kDa). The fimbriae associated protein gene (fap) was strongly expressed in fimbriated A. actinomycetemcomitans 310a but not in non-fimbriated strains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aggregatibacter actinomycetemcomitans / genetics*
Amino Acid Sequence
Bacterial Proteins / genetics*
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA, Bacterial
Fimbriae Proteins*
Fimbriae, Bacterial*
Gene Expression
Genes, Bacterial
Molecular Sequence Data
Sequence Analysis, DNA

Substances

Bacterial Proteins
DNA, Bacterial
fimbriae-associated protein, Actinobacillus
Fimbriae Proteins

Associated data

GENBANK/D83053