Inhibition of tyrosine hydroxylase by bulbocapnine

Planta Med. 1997 Aug;63(4):362-3. doi: 10.1055/s-2006-957702.

Abstract

The inhibitory effects of bulbocapnine, an aporphine isoquinoline alkaloid, on bovine adrenal tyrosine hydroxylase (TH) were investigated. Bulbocapnine had an inhibitory effect on the enzyme (43.6% inhibition at concentration of 200 microM). Bulbocapnine exhibited uncompetitive inhibition on bovine adrenal TH with a substrate L-tyrosine. The Ki value was found to be 0.20 mM.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Glands / drug effects
  • Adrenal Glands / enzymology
  • Animals
  • Aporphines / pharmacology*
  • Cattle
  • Enzyme Inhibitors / pharmacology*
  • Kinetics
  • PC12 Cells
  • Rats
  • Tyrosine 3-Monooxygenase / antagonists & inhibitors*

Substances

  • Aporphines
  • Enzyme Inhibitors
  • Tyrosine 3-Monooxygenase
  • bulbocapnine