A new static alignment method for trans-tibial prostheses is suggested using the individual load line as a reference. Standing posture and static alignment of 18 experienced trans-tibial prosthetic users with good walking ability were determined and compared with 20 healthy persons. The individual load line was defined by means of the new Otto Bock alignment system "L.A.S.A.R. Posture". The sagittal standing posture of trans-tibial amputees and non-amputees differs. Normally only a prosthesis worn by the trans-tibial amputee and dynamically aligned over an extended period of time satisfies biomechanical rules of alignment. In contrast, prostheses aligned during one session in the traditional subjective manner seem to lack any recognizable biomechanical systematics. Initial results suggest the knee centre should be 10 to 30mm behind the load line, depending on patient's weight. This knee position is independent on the type of prosthetic foot.