Propagation and titration of murine cytomegalovirus in a continuous bone marrow-derived stromal cell line (M2-10B4)

J Virol Methods. 1997 Nov;68(2):193-8. doi: 10.1016/s0166-0934(97)00126-2.

Abstract

Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of small amounts of MCMV reactivating from latently infected spleen explants. M2-10B4 cells will be very useful for studies of MCMV pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone Marrow Cells / virology*
  • Cell Line
  • Cytopathogenic Effect, Viral
  • Female
  • Herpesviridae Infections / virology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Muromegalovirus / growth & development*
  • Muromegalovirus / isolation & purification
  • Muromegalovirus / physiology
  • Organ Culture Techniques
  • Spleen / virology
  • Stromal Cells / virology
  • Viral Plaque Assay
  • Virus Latency