Abstract
Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of small amounts of MCMV reactivating from latently infected spleen explants. M2-10B4 cells will be very useful for studies of MCMV pathogenesis.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Bone Marrow Cells / virology*
-
Cell Line
-
Cytopathogenic Effect, Viral
-
Female
-
Herpesviridae Infections / virology
-
Mice
-
Mice, Inbred BALB C
-
Mice, Inbred C3H
-
Mice, Inbred C57BL
-
Muromegalovirus / growth & development*
-
Muromegalovirus / isolation & purification
-
Muromegalovirus / physiology
-
Organ Culture Techniques
-
Spleen / virology
-
Stromal Cells / virology
-
Viral Plaque Assay
-
Virus Latency