Abstract
Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-beta-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Echerichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with Ki of 0.23 and 0.19 microM, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins*
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Enzyme Inhibitors / pharmacology*
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Escherichia coli / genetics
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Kinetics
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Recombinant Proteins / antagonists & inhibitors
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Recombinant Proteins / genetics
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Serratia marcescens / enzymology*
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Sulfhydryl Compounds / pharmacology*
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beta-Lactamase Inhibitors*
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beta-Lactamases / genetics
Substances
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Bacterial Proteins
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Enzyme Inhibitors
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Recombinant Proteins
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Sulfhydryl Compounds
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beta-Lactamase Inhibitors
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beta-Lactamases