Polypyrrole DNA chip on a silicon device: example of hepatitis C virus genotyping

Anal Biochem. 1998 Jan 15;255(2):188-94. doi: 10.1006/abio.1997.2462.

Abstract

We describe in this article an oligonucleotide array constructed on a silicon device bearing a matrix of addressable 50-microns microelectrodes. Each electrode was covered by a conducting polymer (polypyrrole) grafted by an oligonucleotide (ODN). The DNA chip was prepared by successive electrochemically addressed copolymerizations of 5' pyrrole-labeled ODN and pyrrole. Following hybridization of the biotinylated amplified sample on the chip bearing a series of probes, detection was carried out by fluorescence microscopy through an R-phycoerythrin label. This technology was successfully applied to the genotyping of hepatitis C virus in blood samples. Results show good sensitivity and a high degree of dimensional resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotechnology / instrumentation*
  • DNA / chemistry*
  • DNA / genetics
  • DNA, Viral / analysis*
  • Electrochemistry
  • Genotype
  • Hepacivirus / genetics*
  • Microelectrodes
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemistry
  • Pyrroles / chemistry*
  • Quality Control
  • Sensitivity and Specificity
  • Silicon*

Substances

  • DNA, Viral
  • Oligonucleotides
  • Pyrroles
  • DNA
  • Silicon