Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups

M Manzano; L Cocolin; C Pipan; E Falasca; G A Botta; C Cantoni; G Comi

doi:10.1006/mcpr.1997.0137

Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect different serogroups

Mol Cell Probes. 1997 Dec;11(6):459-62. doi: 10.1006/mcpr.1997.0137.

Authors

M Manzano¹, L Cocolin, C Pipan, E Falasca, G A Botta, C Cantoni, G Comi

Affiliation

¹ Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.

PMID: 9500812
DOI: 10.1006/mcpr.1997.0137

Abstract

PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups.

MeSH terms

Bacterial Proteins / genetics*
DNA Primers
Genetic Variation
Humans
Listeria monocytogenes / classification*
Listeria monocytogenes / genetics*
Polymerase Chain Reaction / methods
Polymorphism, Single-Stranded Conformational*
Reproducibility of Results
Serotyping / methods

Substances

60 kDa protein, Listeria monocytogenes
Bacterial Proteins
DNA Primers