Abstract
To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis- active DNA elements (SP1, CREB, C/EBP, AP1 and AP-2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Artificial Gene Fusion
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Base Sequence
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COS Cells
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Cloning, Molecular
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Cytokines / genetics*
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Cytokines / metabolism
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DNA / genetics
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DNA Primers / genetics
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Gene Expression Regulation
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Genes, Reporter
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Humans
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Luciferases / genetics
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Molecular Sequence Data
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Deletion
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Transfection
Substances
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Cytokines
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DNA Primers
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Recombinant Fusion Proteins
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DNA
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cardiotrophin 1
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Luciferases