Cloning and characterization of the 5'-flanking region of the human cardiotrophin-1 gene

Biochem Biophys Res Commun. 1998 Mar 17;244(2):494-7. doi: 10.1006/bbrc.1998.8311.

Abstract

To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis- active DNA elements (SP1, CREB, C/EBP, AP1 and AP-2 like and GATA) in the proximal 1.1 kb region. Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Artificial Gene Fusion
  • Base Sequence
  • COS Cells
  • Cloning, Molecular
  • Cytokines / genetics*
  • Cytokines / metabolism
  • DNA / genetics
  • DNA Primers / genetics
  • Gene Expression Regulation
  • Genes, Reporter
  • Humans
  • Luciferases / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Deletion
  • Transfection

Substances

  • Cytokines
  • DNA Primers
  • Recombinant Fusion Proteins
  • DNA
  • cardiotrophin 1
  • Luciferases

Associated data

  • GENBANK/AJ002743