Role of nucleosome remodeling factor NURF in transcriptional activation of chromatin

Mol Cell. 1997 Dec;1(1):141-50. doi: 10.1016/s1097-2765(00)80015-5.

Abstract

The Drosophila nucleosome remodeling factor (NURF) is a protein complex of four subunits that assists transcription factor-mediated perturbation of nucleosomes in an ATP-dependent manner. We have investigated the role of NURF in activating transcription from a preassembled chromatin template and have found that NURF is able to facilitate transcription mediated by a GAL4 derivative carrying both a DNA binding and an activator domain. Interestingly, once nucleosome remodeling by the DNA binding factor is accomplished, a high level of NURF activity is not continuously required for recruitment of the general transcriptional machinery and transcription for at least 100 nucleotides. Our results provide direct evidence that NURF is able to assist gene activation in a chromatin context, and identify a stage of NURF dependence early in the process leading to transcriptional initiation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism
  • Adenosine Triphosphate / metabolism
  • Animals
  • Chromatin / genetics*
  • Chromatin / metabolism
  • Chromosomal Proteins, Non-Histone*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / pharmacology
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology
  • Heat Shock Transcription Factors
  • Insect Proteins / genetics
  • Insect Proteins / metabolism*
  • Molecular Chaperones*
  • Nuclear Proteins*
  • Nucleosomes / genetics*
  • Nucleosomes / metabolism
  • Retinoblastoma-Binding Protein 4
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription Factors / pharmacology
  • Transcriptional Activation / drug effects
  • Transcriptional Activation / physiology*

Substances

  • Caf1-55 protein, Drosophila
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Drosophila Proteins
  • GAL4 (1-147) protein, S cerevisiae
  • Heat Shock Transcription Factors
  • Hsf protein, Drosophila
  • ISWI protein
  • Insect Proteins
  • Molecular Chaperones
  • Nuclear Proteins
  • Nucleosomes
  • Retinoblastoma-Binding Protein 4
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Adenosine Triphosphate
  • Adenosine Triphosphatases