Abstract
It is proposed that the genesis of extracellular membrane vesicles in Gram-negative bacteria is a result of cell wall turnover. Peptidoglycan turnover would cause a turgor on the outer membrane, causing the outer membrane to bulge and finally bleb. Mechanical motion would then shear the blebs into the culture medium.
MeSH terms
-
Bacterial Outer Membrane Proteins / analysis
-
Cell Wall / chemistry
-
Cell Wall / metabolism
-
Electrophoresis, Polyacrylamide Gel
-
Gram-Negative Bacteria / chemistry
-
Gram-Negative Bacteria / physiology
-
Gram-Negative Bacteria / ultrastructure*
-
Humans
-
Muramic Acids / analysis
-
Peptides / analysis
-
Porphyromonas gingivalis / chemistry
-
Porphyromonas gingivalis / physiology
-
Porphyromonas gingivalis / ultrastructure*
-
Proteins / analysis
Substances
-
Bacterial Outer Membrane Proteins
-
Muramic Acids
-
Peptides
-
Proteins