Proteins and cells on PEG immobilized silicon surfaces

Biomaterials. 1998 May;19(10):953-60. doi: 10.1016/s0142-9612(98)00026-x.

Abstract

Silicon surfaces were modified by covalent attachment of a self-assembled (SA) polyethylene glycol (PEG) film. Adsorption of albumin, fibrinogen, and IgG to PEG immobilized silicon surfaces was studied by ellipsometry to evaluate the non-fouling and non-immunogenic properties of the surfaces. The adhesion and proliferation of human fibroblast and Hela cells onto the modified surfaces were investigated to examine their tissue biocompatibility. Coated PEG chains showed the effective depression of both plasma protein adsorption and cell attachment to the modified surfaces. The mechanisms accounting for the reduction of protein adsorption and cell adhesion on modified surfaces were discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Adhesion / drug effects
  • Cell Division / drug effects
  • Cells, Immobilized
  • Fibrinogen / chemistry*
  • Fibroblasts
  • HeLa Cells
  • Humans
  • Immunoglobulin G / chemistry*
  • Polyethylene Glycols / chemistry*
  • Serum Albumin, Bovine / chemistry*
  • Silicon / chemistry*
  • Surface Properties

Substances

  • Immunoglobulin G
  • Serum Albumin, Bovine
  • Polyethylene Glycols
  • Fibrinogen
  • Silicon